CRISPR technology: A decade of genome editing is only the beginning
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Science. 2023 Jan 20;379(6629):eadd8643. doi: 10.1126/science.add8643. Epub 2023 Jan 20. PMID: 36656942.

CRISPR technology: A decade of genome editing is only the beginning

Authors: Joy Y Wang 1 2Jennifer A Doudna 1 2 3 4 5 6 7 8

Affiliation:

  1. Department of Chemistry, University of California, Berkeley, Berkeley, CA, USA.
  2. Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA.
  3. Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, USA.
  4. Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
  5. California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, USA.
  6. Molecular Biophysics & Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  7. Gladstone Institutes, University of California, San Francisco, San Francisco, CA, USA.
  8. Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA, USA

Abstract:

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome editing, coupled with advances in computing and imaging capabilities, has initiated a new era in which genetic diseases and individual disease susceptibilities are both predictable and actionable. Likewise, genes responsible for plant traits can be identified and altered quickly, transforming the pace of agricultural research and plant breeding. In this Review, we discuss the current state of CRISPR-mediated genetic manipulation in human cells, animals, and plants along with relevant successes and challenges and present a roadmap for the future of this technology.

Download: https://www.science.org/doi/10.1126/science.add8643?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmedv